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Fig. 5 | Molecular Cancer

Fig. 5

From: N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway

Fig. 5

N-Myc-induced up-regulation of ATM promotes migration/invasion of C4–2 cells. a Representative images of migration/invasion capacity for C4–2/Vec and C4–2/N-Myc cells passing through a Trans-well barrier for 48 h. Bottom panel showed the quantification of relative migrated cell number. b and c representative images of C4–2/Vec and C4–2/N-Myc cells migration treated by either miR-421 overexpression by lentivirus infection or ATM inhibitor (10 μM), and corresponding quantifications. d Immunoblot to show endogenous EZH2 protein level of LNCaP and C4–2 cells and knockdown by siRNA (siEZH2) in C4–2/Vec and C4–2/N-Myc cells. GAPDH was used as a loading control. e RT-qPCR to show the increased level of miR-421 in C4–2/N-Myc cells instead of decreased after EZH2 siRNA knockdown. * p < 0.05, ** p < 0.005

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