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Fig. 3 | Molecular Cancer

Fig. 3

From: Novel long noncoding RNA OTUD6B-AS1 indicates poor prognosis and inhibits clear cell renal cell carcinoma proliferation via the Wnt/β-catenin signaling pathway

Fig. 3

Overexpression of OTUD6B-AS1 markedly suppressed the proliferation of ccRCC cells in vitro. a OTUD6B-AS1 expression levels in the ccRCC cell lines (786-O, Caki-1769-P, OS-RC-2 and ACHN) compared with that in the human renal tubular epithelial cell line (HK-2). b ACHN and OS-RC-2 cells treated with 5 μM 5-aza-CdR. c qRT-PCR analysis of the OTUD6B-AS1 expression in the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. (d, e) MTT cell proliferation assays performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. (f, g) Colony formation assays performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. h Cell immunofluorescence staining assay performed with the ACHN and OS-RC-2 cells transfected with plvx-OTUD6B-AS1 or the empty vector. *P < 0.05, **P < 0.01, *** P < 0.001

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