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Fig. 2 | Molecular Cancer

Fig. 2

From: Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Fig. 2

Suppression of circRNA 100146 expresssion inhibits cancer cell proliferation and promotes apoptosis in vitro and inhibits subcutaneous tumor growth in vivo. a, b After knockdown of circRNA 100146, proliferation of 16HBE-T (a) and H460 cells (b) was detected with the EdU assay. Blue fluorescence represents DAPI-stained nuclei and red fluorescence represents EdU-labeled proliferative cells (magnification× 200). c The histogram shows the relative proportions of EdU-positive 16HBE-T and H460 cells. Cells were counted using Image-pro-plus software. d Apoptosis of cancer cells was detected using flow cytometry. Upper left quadrant (UL) represents necrotic cells, lower left quadrant (LL) living cells, right upper quadrant (UR) late apoptotic and necrotic cells, lower right quadrant (LR) early apoptotic cells. e Percentage of apoptotic cells (sum of LR and UR) in the negative control and interference groups. f, g H460-empty vector(f) and H460-sh-circRNA(g) were injected subcutaneously in nude mice . Subcutaneous tumor fluorescence intensities were determined in vivo by using an small animal imaging system at 1, 3, 5, 7, 14, and 21d. There is a fluorescence value coordinate on the right side of the displayed picture, and the darker of the red, the higher of the fluorescence value. h Changes in subcutaneous fluorescence intensity in the empty vector and experimental groups at different time-points were analyzed. i Immunohistochemical detection of expression of PCNA, Caspase-9, E-cadherin and p53 in H460-empty vector and H460 sh-circRNA groups (magnification × 400). c,e Data are presented as means±s.d., n = 3, unpaired t-tests; compared with the siRNA NC group, *p < 0.05, **p < 0.01

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