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Fig. 3 | Molecular Cancer

Fig. 3

From: Circular RNA 100146 functions as an oncogene through direct binding to miR-361-3p and miR-615-5p in non-small cell lung cancer

Fig. 3

CircRNA 100146 binding to miR-361-3p and miR-615-5p indirectly affecting multiple downstream mRNAs expression. a Subcellular localization of circRNA 100146 detected by FISH. Images were obtained under a confocal fluorescence microscope. Green fluorescence represents 6-FAM-labeled circRNA probes while blue fluorescence represents DAPI-stained nuclei. b Sequence alignments between miR-361-3p, miR-615-5p or miR-330-3p, and the seed sequence of circRNA 100146. WT and Mut represent wild-type and mutant sequences of circRNA. c Results of the dual-luciferase reporter gene assay in HEK-293 T cells. d, f Western blot of NFAT5, COL1A1, TRAF3 and MEF2C in 16HBE-T (d) and H460 (f) following interference with circRNA 100146 expression. e Gray value analysis of the protein bands in (d). g Gray value analysis of the protein bands in (f). h Sequence alignments between miRNAs and seed sequence of the 3’-UTR of SF3B3. i Results of the dual-luciferase reporter gene assay. j Expression of SF3B3 in subcutaneous tumors tissues detected using immunohistochemistry. c, i Data are presented as means ± s.d, n = 3, unpaired t-test, compared with the mimic NC + WT group, *p < 0.05, **p < 0.01. e, g Data are presented as means ± s.d, n = 3, unpaired t-test, Compared with siRNA NC group, *p < 0.05, **p < 0.01

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