Fig. 2

Knockdown of circKIF4A inhibits proliferation and metastasis of TNBC a si-circKIF4A #2 successfully knocked down circKIF4A. b A CCK-8 assay to detect cell proliferation. c A colony formation assay to detect cell colony-forming ability. d Colony formation number was quantified by ImageJ. e A Transwell assay to assess cell migratory ability. f The number of invasive cells was quantified by ImageJ. g A wound-healing assay to assess cell migratory capability. h Wound closure was quantified by ImageJ. i-j Immunofluorescence staining of E-cadherin and vimentin. k Xenograft models were established. l Summary of tumor weights. m Representative image of luciferase signals of lung metastatic nodules. n Representative images of lung metastatic nodules and HE-stained sections. o The number of metastatic nodules was quantified. **P < 0.01