Fig. 2

The effect of PVT1 on GBC cells biological behavior in vitro. (a-b) PVT1 expression was knocked down by three siRNAs targeting PVT1 in GBC-SD and NOZ cells. (c-d) Knockdown of PVT1 significantly decreased cell proliferation compared with si-NC cells using CCK-8 assay. (e-f) Colony numbers of GBC-SD and NOZ cells transfected with si-PVT1 were significantly lower than in those transfected with si-NC. (g-h) EdU assay showed that suppression of PVT1 attenuated the proliferation of GBC-SD and NOZ cells (magnification, × 100). Scale bar, 100 μm. (i-l) PVT1 suppression impaired GBC cell invasion and migration, as measured by a transwell assay (magnification, × 100). Scale bar, 100 μm and wound healing assay, respectively. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate mean ± SD