Fig. 8

PVT1 promotes tumor growth in vivo. (a) Images of tumor formation were performed by a live imaging system detecting the luciferase signal. (b) The luciferase activity in the lenti-sh-PVT1 group was lower than in the lenti-sh-NC group. (c-d) The volumes and weight of lenti-sh-PVT1 cell-derived xenograft tumors were markedly lower than those of the lenti-sh-NC group. (e-g) Sections of xenograft tumors stained with hematoxylin and eosin (H&E) as well as immunohistochemical staining for HK2 and Ki-67 (magnification, × 200). Scale bar, 100 μm. (h) Representative images of tumor formation of the lenti-NC group and lenti-PVT1 group. (i) The luciferase activity in the lenti-PVT1 group was higher than that in the lenti-NC group. (j-k) The volumes and weight of lenti-PVT1 cell-derived xenograft tumors were markedly higher than those of the lenti-NC group. (l-n) The levels of Ki67 and HK2 were much higher after PVT1 overexpression (magnification, × 200). Scale bar, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate mean ± SD