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Fig. 3 | Molecular Cancer

Fig. 3

From: Exosomal FMR1-AS1 facilitates maintaining cancer stem-like cell dynamic equilibrium via TLR7/NFκB/c-Myc signaling in female esophageal carcinoma

Fig. 3

Effects of ectopic FMR1-AS1 expression on female ESCC cells. a Cell proliferation assay on FMR1-AS1-upregulated, FMR1-AS1-downregulated and respective control ESCC cells using the Cell Counting Kit-8 (mean ± SD, n = 6, *p < 0.05). b Apoptosis rates on ESCC cells transfected with FMR1-AS1, Control, sh-Control, sh-FMR1-AS1 #1 and sh-FMR1-AS1 #2 lentiviruses, using flow cytometry (mean ± SD, n = 6, *p < 0.05). c, d Migration and invasion of ESCC cells transfected with FMR1-AS1, Control, sh-Control, sh-FMR1-AS1 #1 and sh-FMR1-AS1 #2 lentiviruses. Right panel is the quantification of migrated/invaded cells (mean ± SD, n = 6, *p < 0.05). e Xenograft mice subcutaneously implanted FMR1-AS1-upregulated, FMR1-AS1-downregulated and respective control ESCC cells (mean ± SD, n = 6, *p < 0.05). f Ki67 immunostaining in female ESCC samples and FMR1-AS1 expression in Ki67 strong and weak samples. g Ki67 immunostaining in xenograft tumors of FMR1-AS1-upregulated, FMR1-AS1-downregulated and respective control ESCC cells. h TUNEL staining in xenograft tumors of FMR1-AS1-upregulated, FMR1-AS1-downregulated and respective control ESCC cells

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