Fig. 2
From: Circular RNA cTFRC acts as the sponge of MicroRNA-107 to promote bladder carcinoma progression
The effects of cTFRC on BC cell invasion. a Scheme illustrating the production of cTFRC. b The relative cTFRC level in BC cells. c Transwell assay different BC cells invasion activity. d Comparison of the abundance of cTFRC in the nuclear and cytoplasmic. Fractionation of EJ and T24 cells followed by qPCR. U1 RNA served as a positive control for nuclear gene expression. e RNA FISH for cTFRC. cTFRC probes were labeled with Cy3. Nuclei were stained with DAPI. Scale bar, 10 μm. f qPCR analysis of the transfection efficiency of shcTFRC vectors after transfection in EJ or T24 cells. g Determination of cell invasive potential of EJ, T24 or 5637 cells transfected with shcTFRC by Transwell assay. Data are the means ± SD of three independent experiments. *P < 0.05; **P < 0.01