Fig. 6

cTFRC mediates TGFβ-induced EMT. a-b qRT-PCR or western blot analysis of cTRFC, TFRC and E-cadherin levels: the protein expression of E-cadherin was reduced, while the expressions of cTFRC and TFRC were increased in EJ cells treated with TGF-β or LiCl, in comparison with those at 0 mM (control). β-actin was used as an internal control. c Phase contrast images of EJ or T24 cells 72 h after cTFRC-Vector transfection, showing an EMT-like morphology in cTFRC treated cells. d Western blot analysis of TFRC expression in overexpression cTFRC EJ or T24 cells. e TFRC and E-cadherin expression in shcTFRC and shCtrl-treated EJ or T24 groups. f TFRC and E-cadherin expression in shTFRC and shCtrl-treated EJ or T24 groups. β-actin was used as an internal control