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Fig. 7 | Molecular Cancer

Fig. 7

From: Circular RNA cTFRC acts as the sponge of MicroRNA-107 to promote bladder carcinoma progression

Fig. 7

cTFRC targets miR-107 and inhibits its activity. a Binding sites of miR-107 in 3’UTR of cTFRC and TFRC. b EJ or T24 cells lysates were subject to RNA pull-down assay and tested by RT–PCR. Relative level of miR-107 was normalized to input. miR-107 can be pulled down by cTFRC probe. c Lysates prepared from EJ or T24 cells were subject to RNA pull-down assay and tested by RT–PCR. Relative expression of cTFRC was normalized to input. cTFRC can be pulled down by miR-107 probe. d miR-107 colocalized with cTFRC in BC primary tumor cells and adjacent normal cells were detected by FISH. Data are from three independent experiments. Magnification, 400X. e TFRC can be pulled down by miR-107 probe in EJ or T24 cells. Data are shown as mean ± SD. **P < 0.01 by two-tailed Student’s t test. Data represent at least three independent experiments

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