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Fig. 1 | Molecular Cancer

Fig. 1

From: Nuclear lncRNA HOXD-AS1 suppresses colorectal carcinoma growth and metastasis via inhibiting HOXD3-induced integrin β3 transcriptional activating and MAPK/AKT signalling

Fig. 1

The down-regulation of nuclear HOXD-AS1 predicts poor outcome in CRC patients. a Expression levels of HOXD-AS1 in 35 paired CRC and adjacent non-cancerous tissues. b Expression levels of HOXD-AS1 in CRC and colon mucosa epithelial (FHC) cell lines. c The proportion of HOXD-AS1 in the cytoplasmic and nuclear fraction in CRC and FHC cells were detected by real-time PCR. HOXD-AS1 expression was normalized to GAPDH (exported to cytoplasm) and U6 (nuclear retained). d-e The analysis of HOXD-AS1 expression in CRC compared with normal tissues in CRC microarray profile (GES8671, Paired t test; GSE21510, Mann-Whitney test) and RNA-seq in TCGA database. f Expression analysis of HOXD-AS1 in normal colorectal mucosa and CRC tissues by ISH. Scale bars, 50 μm (200×) or 20 μm (400×), were shown in the right corner of each picture. g Graphical illustration of statistical HOXD-AS1 distribution in CRC patients. h Kaplan–Meier analysis of overall survival in all patients with CRC according to HOXD-AS1 expression. (log-rank test). For a-c, data are presented as means ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598