Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: Nuclear lncRNA HOXD-AS1 suppresses colorectal carcinoma growth and metastasis via inhibiting HOXD3-induced integrin β3 transcriptional activating and MAPK/AKT signalling

Fig. 2

HOXD-AS1 suppresses CRC cell proliferation, cell-cycle progression, migration and invasion in vitro. a HOXD-AS1-overexpressing SW620 and M5 cell lines were established by the transfection of pcDNA3.1-HOXD-AS1. And infecting SW480 and DLD-1cells with a lentivirus vector harbouring shRNA-HOXD-AS1 to knocked down the endogenous expression of HOXD-AS1 in cells. HOXD-AS1 levels in cells were detected by real-time PCR. b CCK-8 assays were performed to determine the proliferation of HOXD-AS1-overexpressed or -depleted CRC cells. c Colony-forming assays were performed to determine the effects of HOXD-AS1-overexpression or -depletion on the growth of CRC cells. The diameter > 50 cells was scored. d Cell cycle progression was analyzed by flow cytometry. e The migration potencies of CRC cells with the indicated treatments were detected by using wound healing assay. f Invasion assays were used to determine the effects of HOXD1-overexpression or -depletion on the invasion ability of CRC cells. For a-f, data were expressed as means ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Molecular Cancer

ISSN: 1476-4598