Fig. 5

Over-expression of circPSMC3 regulates PTEN expression and inhibits GC cells activities by targeting miR-296-5p. a The relative luciferase activities were analyzed in 293 T cells co-transfected with circPSMC3 vector or Mock and miR-296-5p mimics or miR-NC and luciferase reporters vectors psiCHECK2- PTEN 3 UTR-WT or psiCHECK2-PTEN 3 UTR-Mut. b The expression levels of PTEN mRNA were analyzed using RT-qPCR in MGC803 and AGS cells were co-transfected withcircPSMC3 vector or Mock and miR-296-5p mimics or miR-NC. c The expression levels of PTEN protein were analyzed using Western blot in MGC803 and AGS cells were co-transfected with circPSMC3 vector or Mock and miR-296-5p mimics or miR-NC. d The growth curves of cells were measured after co-transfected with circPSMC3 and miR-296-5p mimics by using CCK-8 assays. e EdU assays of GC cells co-transfected with circPSMC3 and miR-296-5p mimics were performed to evaluate cell proliferation. f The cell apoptosis ability was evaluated by using apoptosis assay in cells co-transfected with circPSMC3 and miR-296-5p mimics. g Cell motility was examined in cells co-transfected with circPSMC3 and miR-296-5p mimics by wound healing assay. h Cell invasion or migration assays were performed in cells co-transfected with circPSMC3 and miR-296-5p mimics by using transwell chamber with or without matrigel respectively. Data indicate mean ± SD of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, Scale bar, 100 mm