Table 2 RT-PCR primers
From: Hypoxic BMSC-derived exosomal miRNAs promote metastasis of lung cancer cells via STAT3-induced EMT
Gene | Forward primer (5′- to 3′) | Reverse primer (5′- to 3′) |
|---|---|---|
E-cadherin | CAGGTCTCCTCATGGCTTTGC | CTTCCGAAAAGAAGGCTGTCC |
N-cadherin | AGCGCAGTCTTACCGAAGG | TCGCTGCTTTCATACTGAACTTT |
Vimentin | CGTCCACACGCACCTACAG | GGGGGATGAGGAATAGAGGCT |
Fibronectin | ATGTGGACCCCTCCTGATAGT | GCCCAGTGATTTCAGCAAAGG |
Snail | CACACGCTGCCTTGTGTCT | GGTCAGCAAAAGCACGGTT |
Twist | GGACAAGCTGAGCAAGATTCA | CGGAGAAGGCGTAGCTGAG |
Zeb1 | GCTGGCAAGACAACGTGAAAG | GCCTCAGGATAAATGACGGC |
Slug | TGGTCAAGAAACATTTCAACGCC | GGTGAGGATCTCTGGTTTTGGTA |
β-actin | GTGCTATGTTGCTCTAGACTTCG | ATGCCACAGGATTCCATACC |