Fig. 7

circAKT3 regulates PIK3R1 expression, induces cisplatin resistance and activates the PI3K/AKT signaling cascade by targeting miR-198 in vitro. a & b The expression levels of PIK3R1 were analyzed using RT-qPCR. SGC7901CDDP cells were transfected with indicated vectors alone or cotransfected the inhibitors, and SGC7901 cells were transfected with the indicated vectors and miR-198 mimics. c The IC50 of circAKT3 was analyzed by the CCK8 assay. SGC7901CDDP cells were transfected with inhibitor alone or cotransfected with the indicated vectors upon CDDP exposure (6 μM) for 48 h. d The apoptosis rates of SGC7901CDDP cells transfected with indicated vectors alone or cotransfected the inhibitors upon CDDP exposure (6 μM) for 48 h. e The expression levels of PIK3R1, apoptosis markers, γH2AX, BRCA1 and PI3K/AKT signaling molecules were determined using western blotting in SGC7901CDDP cells transfected with indicated vectors alone or cotransfected with the inhibitor after CDDP treatment (6 μM). f The expression levels of PIK3R1, apoptosis markers, γH2AX, BRCA1 and PI3K/AKT signaling molecules were determined using western blotting in SGC7901 cells transfected with the indicated vectors and miR-198 mimics after CDDP treatment (1.5 μM). Proteins were isolated from the indicated cells. g The expression levels of PIK3R1, apoptosis makers, γH2AX, BRCA1 and PI3K/AKT signaling molecules were determined using western blotting in SGC7901 cells transfected with the indicated vectors and BKM20 after CDDP treatment (1.5 μM). The results are presented as the mean ± SEM. *P < 0.05, **P < 0.01