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Fig. 1 | Molecular Cancer

Fig. 1

From: Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis

Fig. 1

CD163+ TAMs at invasive front is correlated with EMT phenotype, MCTC ratio, and poor prognosis in CRC patients. (a) Representative IHC staining for CD68, CD163, E-cadherin, and Vimentin in the invasive front and non-invasive front of serial sections from a human CRC sample. (b-c) Expression of E-cadherin and Vimentin in human CRC samples with low or high CD68 and CD163 expression at invasive front, respectively. (d) Representative CTC images from included patient 5 and 27, respectively. Four-color immunocytochemistry method based on FITC-labeled anti-CK, PE-labeled anti-Vimentin, AF647-labeled anti-CD45, and Hoechst nuclear staining was applied to identify and enumerate CTCs from non-specially trapped WBCs. Scale bar, 20 μm. (e-f) Association of CD68 and CD163 expression at invasive front witth MCTC ratio, respectively. (g-h) Association of CD68 expression at invasive front with the patients’ recurrence-free survival and overall survival in CRC, respectively. (i-j) Association of CD163 expression at invasive front with the patients’ recurrence-free survival and overall survival in CRC, respectively. Error bars, SEM. ns, not significant; ***P < 0.001

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