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Fig. 2 | Molecular Cancer

Fig. 2

From: Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis

Fig. 2

CD163+ TAMs induce EMT to promote migration and invasion of CRC cells. (a) Schema for representing the experiment procedures. (b) PMA-treated THP-1 macrophages were cultured with NCM460-, HCT116- or HT29-conditioned media for 48 h. The representative bright-field images of macrophages treated by the respective conditioned media are shown. (magnification, ×200). (c) RT-PCR analyzed the expression of the markers of pan-macrophage (CD68), M1 (arginase 1, CD86, HLA-DR) and M2 (CD163, CD206) macrophages in PMA-treated THP-1 macrophages incubated with the conditioned media from NCM460, HCT116 and HT29 for 48 h; Error bars, SEM. (d) The effect of the TAMs on the EMT of CRC cells (HCT116 and HT29) was analyzed by Western blot analysis. (e) RT-PCR for analyzing the expression of E-cadherin and Vimentin in CRC cells (HCT116 and HT29) alone or co-cultured with macrophages (PMA-treated THP-1 macrophages or TAMs) for 48 h; Error bars, SEM. (f), (g) and (h) Cell proliferation, migration and invasion capacity of CRC cells (HCT116 and HT29) alone or co-cultured with macrophages (PMA-treated THP-1 macrophages or TAMs) was determined by the colony formation, wound healing assay and transwell coculture system, respectively. Representative photographs of migratory or invaded cells (magnification, × 200) are shown; Error bars, SD. **P < 0.01; ***P < 0.001

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