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Fig. 3 | Molecular Cancer

Fig. 3

From: Crosstalk between cancer cells and tumor associated macrophages is required for mesenchymal circulating tumor cell-mediated colorectal cancer metastasis

Fig. 3

IL6 is required for TAMs-induced EMT of CRC cells. (a) Relative expression levels of representative EMT/inflammation related-cytokines were detected in TAMs co-cultured with HCT116 as determined by RT-PCR; Error bars, SEM. (b) ELISA assay of IL6 protein secretion of CRC cells (HCT116 and HT29) and various macrophages; Error bars, SD. (c) IL6 mRNA expression in HCT116 and TAMs with or without 48 h of coculture. Error bars, SEM. (d) Expression of EMT markers E-cadherin and Vimentin in CRC cells (HCT116 and HT29) alone, IL6-supplemented CRC cells, TAMs-co-cultured CRC cells, and IL6 depleted TAMs-co-cultured CRC cells were analyzed by Western blot. (e) Colony formation assay was used to quantify the number of spheres of IL6 depleted TAMs-co-cultured CRC cells (HCT116 and HT29) and its control; Error bars, SD. (f) Migration of IL6 depleted TAMs-co-cultured CRC cells (HCT116 and HT29) and its control was measured by wound-healing assay. Error bars, SD. (g) Invasion of IL6 depleted TAMs-co-cultured CRC cells (HCT116 and HT29) and its control was measured by transwell (magnification, × 200); Error bars, SD. ***P < 0.001

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