Fig. 5

MiR155 modulated PD-1/PD-L1-mediated B-lymphoma cell interaction with CD8+T cells via upregulating PD-L1 expression. a Bioinformatics analysis predicted potential binding sites of miR155 with the 3′-UTR of PD-L1. b The effect of miR155 on transcriptional activity of PD-L1 3′-UTR was detected by luciferase reporter assay in HEK-293T cells transfected with control mimics or miR155 mimics. c PD-1/PD-L1 interaction between lymphoma cells and CD8+T cells in the co-culture systems (Representative immunofluorescene images of PD-L1[green])/PD-1[red] with nucleus counterstained with DAPI [blue]). d and e Phosphorylated AKT and ERK were detected by western blot in CD8+T cells sorted from the co-culture system of Farage cells (upper panel) and DB cells (lower panel)