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Fig. 5 | Molecular Cancer

Fig. 5

From: RETRACTED ARTICLE: Exosome-transmitted miR-128-3p increase chemosensitivity of oxaliplatin-resistant colorectal cancer

Fig. 5

Intercellular transfer of miR-128-3p by 128-Exo sensitizes CRC cells to oxaliplatin agents. a CCK8 assay of HCT116OxR cells pre-incubated with indicated factors for 48 h followed by oxaliplatin treatment at indicated concentrations. b Flow cytometry for apoptosis assay of HCT116OxR cells pre-incubated with indicated factors for 48 h followed by oxaliplatin treatment (30 μM) for 24 h. c Western blot analysis of protein E-cad, N-cad, Vim, and Fn expression in HCT116OxR cells after incubated with indicated factors. d Migration and invasion ability of HCT116OxR cells after incubated with indicated factors for 48 h were assessed by Transwell assays. e Motility ability of HCT116OxR cells after incubated with indicated factors for 48 h were assayed by wound healing assays. f Accumulation of Pt in HCT116OxR cells after incubated with indicated factors for 48 h following exposure to 30 μM, 24 h oxaliplatin treatment. g Total Pt-DNA adduct levels in HCT116OxR cells after incubated with indicated factors for 48 h following exposure to 30 μM, 24 h oxaliplatin treatment. h Immunofluorescence analysis of nuclear foci for γ-H2AX expression HCT116OxR cells incubated with indicated factors for 48 h followed by 24 h` oxaliplatin exposure (30 μM). Scale bars, 10 μm. i Subcutaneous xenograft assay of HCT116OxR cells (5 × 106 cells) in nude mice with intratumoral injection of PBS, 128-exo and exo/FHC miR-128-3p electrop in nude mice with vehicle or oxaliplatin (90 mg/m2) treatment. Tumor volume of xenograft models were measured from day 0 to day 42. Volumes of tumors (i) and representative bioluminescent images (j) are shown (n = 5 per group). Results are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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