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Fig. 1 | Molecular Cancer

Fig. 1

From: p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma

Fig. 1

Downregulation of LincRNA-p21 is observed in HNSCC and indicates poor prognosis. a The expression of lincRNA-p21 was detected using an RNAscope® in situ assay in normal oral mucosa tissue and OSCC tissues. The red triangles indicate the lincRNA-p21 blots. Scale bars, 20 μm. b The lincRNA-p21 staining score in HNSCC patients and normal controls was quantitatively analyzed. c-f The lincRNA-p21 staining score was analyzed according to pathological differentiation grade, TNM stage, gender and age in HNSCC patients. g Survival analysis was performed in HNSCC dataset from TCGA database (http://www.cbioportal.org/). h LincRNA-p21 expression was detected in tumor (T) and paracancerous normal tissues (P) from 73 HNSCC patients by qPCR. i LincRNA-p21 expression was measured in HNSCC cell lines and normal oral keratinocytes. j Localization of lincRNA-p21 was analyzed by PCR in Cal27 cells. U6 RNA and β-actin were used as positive controls for nuclear RNA and cytoplasmic RNA, respectively. k The distribution of lincRNA-p21 was analyzed by PCR in HNSCC cell lines. l The distribution of lincRNA-p21 was analyzed by FISH in Cal27 cells. 18S RNA and U6 indicated cytoplasm and nucleus, respectively. Scale bars, 100 μm *: P < 0.05; **: P < 0.01

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