Fig. 3

LincRNA-p21 suppresses HNSCC tumor growth in vitro and in vivo. a Cell viability of HN6 and Cal27 cells after transfection with si-lincRNA-p21 or scrambled was determined using CCK8 assays. b Cell viability of lincRNA-p21- or vector-transfected HN6 and Cal27 cells was determined using CCK8 assays. c A colony formation assay was used to determine the colony formation ability of si-lincRNA-p21- or scrambled-transfected HN6 and Cal27 cells. d The colony formation assay was applied in HN6 and Cal27 cells after transfection with lincRNA-p21 or vector plasmids. e AKT and ERK1/2 signaling was analyzed via western blotting after transfection for 48 h. f, g EdU assays were performed after transfection of HN6 and Cal27 cells for 48 h. Scale bars, 100 μm. h, i Representative images of xenograft tumors derived from si-lentivirus lincRNA-p21, lentivirus lincRNA-p21 or scrambled cells were shown. j The tumor weight after removal from mice in the knockdown, ectopic expressed and scrambled control groups was measured. k The tumor growth curves after cell injection in the si-lentivirus lincRNA-p21, lentivirus lincRNA-p21 or scrambled group were analyzed. l HE and Ki67 staining were detected by immunohistochemistry in the xenograft tumor tissues. Original magnification: × 200. *: P < 0.05; **: P < 0.01