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Fig. 4 | Molecular Cancer

Fig. 4

From: p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma

Fig. 4

LincRNA-p21 regulates cell cycle and induces apoptosis in HNSCC cells. a, b Cell cycle distribution was determined by PI staining after transfection of HN6 and Cal27 cells with siRNA or lincRNA-p21 plasmid for 48 h. c Cell cycle regulation-related proteins were detected in HN6 and Cal27 cells after transfection for 48 h. d The proportion of apoptotic cells was detected after lincRNA-p21 plasmid transfection for 48 h using PI and Annexin V staining. e The proportion of apoptotic cells was detected by TUNEL assays in the xenograft tumor tissues. Scale bars, 100 μm. f PARP, Caspase-3 and its active forms were detected in HN6 and Cal27 cells after transfection for 48 h. g, h Migration and invasion assays were performed with transfected cells using Transwell inserts. i MMP2 and MMP9 expression was detected in HN6 and Cal27 cells after transfection for 48 h. *: P < 0.05; **: P < 0.01

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