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Fig. 5 | Molecular Cancer

Fig. 5

From: p53-targeted lincRNA-p21 acts as a tumor suppressor by inhibiting JAK2/STAT3 signaling pathways in head and neck squamous cell carcinoma

Fig. 5

Knockdown NF-YA expression reverses tumor suppressor activation of lincRNA-p21 in mutant p53 cells, not wild-type p53. a Cell viability was detected using CCK8 in Cal27 (mut-p53) and HN30 (wt-p53) cells after co-transfection with lincRNA-p21 and si-NF-YA for 24 h. b Colony formation was conducted in Cal27 and HN30 cells after co-transfection with lincRNA-p21 and si-NF-YA. c Cleaved PARP and Cyclin D1 was detected using western blot after co-transfection with lincRNA-p21 and si-NF-YA for 48 h in Cal27 and HN30 cells. d Cell cycle distribution was determined by PI staining after co-transfection for 48 h. *: P < 0.05 and **: P < 0.01 indicated the significance compared to the vector control. #: P < 0.05 and ##: P < 0.01 indicated the significance between lincRNA-p21 overexpression groups

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