Fig. 6

LincRNA-p21 inhibits JAK2/STAT3 activation in HNSCC cells. a, b The protein levels of STAT3, p-STAT3, JAK2 and p-JAK2 were detected in HN6 and Cal27 cells transfected with lincRNA-p21 plasmid or si-lincRNA-p21 for 48 h. c The expression of p-STAT3 in HN6 and Cal27 cells transfected with lincRNA-p21 plasmid for 48 h was visualized by immunofluorescence. d The expression of STAT3 and p-STAT3 protein was detected after transfection of HN6 and Cal27 cells for 24 h followed by stimulation with 50 ng/ml IL6 for 30 min. e p-STAT3 was detected by immunohistochemistry in the xenograft tumor tissues. Original magnification: × 200. f The correlation between lincRNA-p21 and p-STAT3 expression in HNSCC tissues was detected and analyzed using RNAscope and immunohistochemistry, respectively. Scale bars, 20 μm and 100 μm. g The expression of STAT3 and p-STAT3 protein were detected after co-transfection with lincRNA-p21 and si-p53 for 48 h. h After treatment with STAT3 inhibitor cryptotanshinone (crypto) for 24 h, STAT3 and p-STAT3 expression were detected by western blot in HN6 and Cal27 cells. i Cell viability was detected using CCK8 after transfection with lincRNA-p21 and incubation with 1 μM cryptotanshinone for 72 h. **: P < 0.01 indicated the significance compared to the control and ^##: P < 0.01 indicated the significance compared to the combination group