Fig. 2

CSCs exosomes accelerate EMT progress of CCRCC cells. a CSCs exosomes induced cell viability of ACHN and 786-O cells. Cell number was counted via MTT assay after adding exosomes. n = 10 for ACHN and 786-O, **P < 0.01 relative to the controls. b CSCs exosomes promoted migration of ACHN and 786-O cells as reported by wound healing assay. Photomicrographs at 200× magnification. n = 4, *P < 0.05, **P < 0.01 relative to the controls. c CSCs exosomes promoted invasion of ACHN and 786-O cells as revealed by transwell assay. n = 4, *P < 0.05, **P < 0.01 relative to the controls. d The expression levels of four EMT-related genes (E-cadherin, N-cadherin, Vimentin, and Twist) in ACHN and 786-O cells were quantified using qRT-PCR. n = 5 for ACHN and 786-O, **P < 0.01 relative to the controls. e The protein levels of four EMT-related genes after treating with varying exosomes determined by western blot. n = 5 for ACHN and 786-O, *P < 0.05, **P < 0.01 relative to the controls. n = 5 for ACHN; n = 5 for E-cadherin, Vimentin and Twist and n = 6 for N-cadherin in 786-O, *P < 0.05, **P < 0.01 relative to the controls