Fig. 4

MiR-19b-3p promotes EMT via targeting PTEN. a The results of wound healing assay showing the effects of infected miR-19b-3p or miR-19b-3p inhibitor (anti-miR) lentivirus on migration of ACHN and 786-O cells respectively. n = 4, **P < 0.01 relative to the controls. NC represents the negative control (same below). b The results of transwell assay showing the effects of miR-19b-3p or anti-miR on invasion of ACHN and 786-O cells. n = 5, **P < 0.01 relative to the controls. c miR-19b-3p expression was quantified using qRT-PCR. n = 5, **P < 0.01 relative to the controls. d qRT-PCR results showing the effects of miR-19b-3p or anti-miR on the expression of E-cadherin, N-cadherin, Vimentin, and Twist in ACHN and 786-O cells. n = 5, **P < 0.01 relative to the controls. e Western blots results showing the effects of miR-19b-3p or anti-miR on protein levels of E-cadherin, N-cadherin, Vimentin and Twist in ACHN and 786-O cells. n = 6 for E-cadherin, Vimentin and Twist and n = 5 for N-cadherin in ACHN; n = 7 for E-cadherin, N-cadherin and Twist and n = 6 for Vimentin in 786-O. *P < 0.05, **P < 0.01 relative to the controls. f Changes of protein levels of PTEN after treatment with varying exosomes in ACHN and 786-O cells. All experiments were repeated at least 3 times with duplicate samples, *P < 0.05; **P < 0.001. g Sequence alignment between PTEN mRNA and miR-19b-3p showing the matched nucleotides in the seed site of miR-19b-3p and the nucleotide-substitution mutation of the seed site of PTEN. h Luciferase reporter gene assay showing the inhibitory effects of miR-19b-3p mimic on luciferase activities in HEK293 cells. NC: The negative control construct of miR-19b-3p mimic. From B to H, n = 5 for all data, *P < 0.05, relative to the control