Fig. 2

ddPCR validation of let-7d-3p and miR-30d-5p as diagnostic markers in 203 independent samples. a Expression and ROC analysis of let-7d-3p in validation samples (P = 1.4e-7 and AUC = 0.822). b Expression and ROC analysis of miR-30d-5p in validation samples (P = 5.4e-7 and AUC = 0.79). c ROC analysis of expression levels of two miRNAs (i.e., let-7d-5p and miR-30d-5p) from sequencing (AUC = 0.922, sequencing samples, n = 121) and ddPCR (AUC = 0.828, validation samples, n = 203). d ROC analysis of 166 validation samples that had at least one cytology test result. miRNA-AUC: ROC analysis based on two miRNAs (i.e., let-7d-5p and miR-30d-5p); cytology-AUC: ROC analysis based on cytology test results; combined-AUC: ROC analysis based on both miRNAs and cytology test results. All ROC analyses were performed to evaluate the sensitivity and specificity of exosomal miRNAs and/or cytology tests to discriminate CIN II+ from CIN I- subjects