Fig. 3
From: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
Silencing FTO inhibited breast tumor growth in nude mice models. a Stable knockdown of FTO in 4 T1 cells by lentiviral FTO shRNA (shFTO). The knockdown effect was verified at the protein levels. b Inhibition of FTO by Rhein in 4 T1 cells with various concentration. c Knockdown and inhibition of FTO effectively suppressed 4 T1 cell growth in mice. 1 × 106 4 T1 cells were subcutaneously implanted in mice. Mice were treated with Rhein or DMSO 3 times per week. d, e The size (d) and the weight (e) of tumor formed in the subcutaneous implantation mice model was monitored every 3 days. **P ≤ 0.01, ****P ≤ 0.0001. f-g Knockdown of FTO dramatically suppressed breast tumor growth in orthotopic xenograft mouse model. Stable FTO-knockdown MDA-MB-231 cells and control cells were injected into the mammary fat pad of each NOD/SCID mouse. 30 days after injection, luciferase activity was measured (f) and quantified (g) by an IVIS device. **P ≤ 0.01. h-j Knockdown of FTO abolished lung metastasis in mice. 4 T1 cells were tail vain injected into BALB/c mice. Formation of breast cancer metastatic foci in the lung was pictured (h) and quantified (i) after 2 weeks. The metastases were confirmed by hematoxylin and eosin staining (j). *P ≤ 0.05