Fig. 5
From: RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3
Epigenetic silencing of BNIP3 by an FTO-m6A-dependent mechanism. a-b BNIP3 expression was significantly up-regulated in both RNA and protein expression level in stable FTO-knockdown MDA-MB-231 cells (a) and MCF-7 cells (b). **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. c, d Knockdown of FTO promoted the cleavage of Caaspase 3 and decreased Bcl2 in MDA-MB-231 cells (c) and MCF-7 cells (d). e Knockdown of FTO promoted the m6A methylation in BNIP3 mRNA by the m6A MeRIP analysis. *P ≤ 0.05. f Wild-type or m6A consensus sequence mutant BNIP3 3’UTR was fused with firefly luciferase reporter. Mutation of m6A consensus sequences were generated by replacing adenosine with thymine. g Relative luciferase activity of the wild-type and 3 mutant BNIP3 3’UTR reporter vectors in FTO-knockdown MDA-MB-231 cells