Fig. 1

circ-DONSON is upregulated in GC tissues and positively correlated with poor prognosis. a Heatmap according to differentially expressed circRNAs between GC tissues and adjacent normal tissues in GSE83521 dataset. b Expression intensity of circ-DONSON in GC tissues and adjacent normal tissues based on GSE83521 dataset. c qRT-PCR analysis of relative circ-DONSON expression levels in 142GC tissues and their adjacent normal tissues. d Northern blotting analysis of circ-DONSON expression in GC tissues and paired normal tissues. e In situ hybridization (ISH) was used to analyze circ-DONSON expression in GC tissues and paired normal tissues. Scale bar: 50 μm. f Increased expression of circ-DONSON was observed in GC cell lines compared to GES-1 cells. g Relative expression of circ-DONSON in GC with different TNM stage. h Expression levels of circ-DONSON in GC patients with or without lymph node metastasis. i, j Kaplan-Meier plots of the overall survival and disease-free survival of GC patients with high (n = 68) and low (n = 74) levels of circ-DONSON. *P < 0.05, **P < 0.01 and ***P < 0.001