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Fig. 5 | Molecular Cancer

Fig. 5

From: Circular RNA circ-DONSON facilitates gastric cancer growth and invasion via NURF complex dependent activation of transcription factor SOX4

Fig. 5

circ-DONSON recruits the NURF complex to activate SOX4 transcription. a ChIP assay showed that SNF2L, BPTF and RBBP4 were enriched on SOX4 promoter. b ChIP assay showed that circ-DONSON silencing attenuated the enrichment of SNF2L, BPTF and RBBP4 on SOX4 promoter. c DNA-FISH verified that circ-DONSON silencing abrogated the colocalization between SNF2L and SOX4 promoter in BGC-823 cells. Scale bar: 5 μm. d, e Depletion of SNF2L, BPTF or RBBP4 impaired the enrichment of active markers H3K27ac and H3K4me3 on SOX4 promoter. f Luciferase reporter assay showed that overexpression of SNF2L, BPTF or RBBP4 increased the luciferase activity while circ-DONSON silencing abrogated it. The SOX4 promoter region was constructed into the pGL3 luciferase vector. g, h qRT-PCR and western blotting analyses of SOX4 expression after knockdown of SNF2L, BPTF or RBBP4. i qRT-PCR analysis indicated that SOX4 expression was negatively correlated with SNF2L in GC tissues. **P < 0.01

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Molecular Cancer

ISSN: 1476-4598