Fig. 2

CircTADA2A affects the migration, invasion, proliferation abilities of osteosarcoma. a The expression of circTADA2A and TADA2A mRNA in HOS and 143B cells after stable transfection of circTADA2A sh-circTADA2A or N.C. (vector plasmids) were detected by qRT-PCR. b Transwell migration and Matrigel invasion assays were used to measure the migration and invasion ability of stable HOS and 143B cells. Representative images are shown; scale bars, 100 μm. c CircTADA2A knockdown suppressed cell migration capacity, as determined by the wound-healing assay. d Colony formation assay was used to determine the ability of proliferation in the stably transfected HOS and 143B cells. e ShRNA-mediated circTADA2A silence suppressed OS cell growth, as determined by the CCK-8 assay. f HOS and 143B cells were transfected with sh-circTADA2A, followed by Annexin V-FITC/PI staining. The percentage of apoptotic cells is shown. Data are from three independent experiments (mean ± SEM) (a-f) (*P < 0.01 vs control or as indicated by Student’s t-test)