Fig. 4

MiR-203a-3p regulates the migration, invasion and proliferation of OS cells. a The expression of miR-203a-3p was detected by qRT-PCR assay in 10 osteosarcoma and chondroma tissues (n = 10) (*P < 0.01, Student’s t-test). b FISH showed the miR-203a-3p expression level was lower in osteosarcoma tissue than in chondroma tissue; scale bars, 200 μm and 50 μm. c MiR-203a-3p expression was detected by qRT-PCR in various human osteosarcoma cell lines (HOS, 143B, U2OS, SJSA-1 and MG63) and normal cells (osteoblast hFOB1.19 and HEK-293). d The expression of miR-203a-3p in OS at different clinicopathological stages was analyzed by FISH on an OS tissue chip (56 cases). Intensity of miR-203a-3p expression was calculated from the OS tissue chip. e The effect of miR-203a-3p on cell migration and invasion was evaluated by Transwell migration and Matrigel invasion assay. Representative images were shown. Scale bars, 100 μm. f Representative images of the wound-healing assay exhibiting changes in the migration capacity of stable OS cells. g Proliferation ability of stably transfected OS cells was evaluated by colony formation assay. Representative images are shown. h pre-miR-203a-3p (or miR-203a-3p sponge)-mediated miR-203a-3p overexpression (or knockdown) influenced the OS cell viability, as determined by a CCK-8 assay. Data are from three independent experiments (mean ± SEM) (c, e-h) or are representative of three independent experiments with similar results (b) (*P < 0.01 vs control or as indicated by Student’s t-test)