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Fig. 7 | Molecular Cancer

Fig. 7

From: Circular RNA circTADA2A promotes osteosarcoma progression and metastasis by sponging miR-203a-3p and regulating CREB3 expression

Fig. 7

CREB3 functions as a driver gene in OS cells. a Western blotting demonstrated the rescue ability of CREB3 on sh-circTADA2A knockdown in HOS and 143B cells. Protein levels of CREB3, c-Jun, Bcl-2, mmp9, mmp2, E-cadherin, N-cadherin and Vimentin were determined. b Effects of circTADA2A knockdown on cell migration and invasion were abrogated by CREB3 overexpression. Migration and invasion of OS cells cotransfected with sh-circTADA2A and CREB3 or control shRNA(N.C.) were evaluated by Transwell migration and Matrigel invasion assays, respectively; scale bars, 100 μm. c The effects of sh-circTADA2A and CREB3 overexpression on cell migration were determined by a wound-healing assay in OS cells. d CREB3 overexpression stimulated the growth of circTADA2A knockdown-mediated cells, as determined by a colony formation assay. e CCK-8 assay demonstrated the cell viability alteration of HOS and 143B cells under the effect of circTADA2A silencing and CREB3 overexpression. f The proliferation ability of stable OS cells was evaluated by soft agar colony formation assay at 0, 7, and 14 days; scale bars, 100 μm. Data are from three independent experiments (mean ± SEM) (b-e) or are representative of three independent experiments with similar results (a and f) (*P < 0.01 vs control or as indicated by Student’s t-test)

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