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Fig. 2 | Molecular Cancer

Fig. 2

From: A novel protein encoded by a circular RNA circPPP1R12A promotes tumor pathogenesis and metastasis of colon cancer via Hippo-YAP signaling

Fig. 2

Characterization the existence and subcellular distribution of circPPP1R12A in CC cells and tissues. a The divergent primers detected circPPP1R12A in cDNA but not in gDNA. b Real-time PCR analysis of circPPP1R12A and linear PPP1R12A mRNA after treatment with RNase R in HCT-116 cells showed that circPPP1R12A was resistant to RNase R treatment. The sub-cellular distribution of circPPP1R12A was mostly present in the cytoplasm by the nuclear mass separation assay (c) and FISH (d). e The level of circPPP1R12A was analyzed by in situ hybridization on CC tissue microarray, showing that circPPP1R12A was up-regulated in CC tissues compared with normal tissues, and such up-regulation was positively correlated with larger tumors and a higher TNM stage. f Kaplan–Meier analysis of the correlation between circPPP1R12A expression and overall survival showed that patients with higher levels of circPPP1R12A had a significantly shorter overall survival. ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598