Fig. 2

Depletion of lncRNA-IUR expression enhances Abl-transformed cell survival and tumor growth in a xenograft mouse model. a, Quantitative real-time PCR was performed to examine lncRNA-IUR expression in K562 cell lines stably expressing sh-IUR-5, -6, -8 or sh-luc (n = 3; means ± SEM; **p < 0.01). b, Cell viability of indicated K562 cell lines was analyzed by flow cytometry upon treatment with indicated concentrations of imatinib for 36 h (n = 3; means ± SEM; **p < 0.01; *p < 0.05). c and d, Nude mice were subcutaneously injected with indicated K562 cell lines. Tumor growth was measured by bioluminescent imaging (c), and then excised from the nude mice (d). Shown were representative images from at least three independent experiments with similar results. e, Quantitative real-time PCR was performed to examine the murine lncRNA-IUR expression in NS2 cell lines expressing sh-IUR-m45678 or sh-luc (n = 3; means ± SEM; **p < 0.01). f, Cell viability of indicated NS2 cell lines was analyzed by flow cytometry after treatment with indicated concentrations of imatinib for 36 h (n = 3; means ± SEM; *p < 0.05). g and h, Nude mice were subcutaneously injected with indicated NS2 cell lines. Tumor growth was measured by bioluminescent imaging (g), and analysis of murine lncRNA-IUR expression in these tumor lysates was measured by quantitative real-time PCR (n = 3; means ± SEM; **p < 0.01; *p < 0.05) (h)