Fig. 5

LncRNA-IUR knockdown transgenic mice provides an ideal microenvironment for Abl-mediated leukemia development. a, Scheme of in vivo leukemia transplant using KD and WT mice. Briefly, lncRNA-IUR KD mice and their WT littermates were infused with GFP-positive NS2 cells or PBS by vena caudalis after sub-lethally irradiation. Experimental groups were labeled as NS2-KD and NS2-WT, and control groups were labeled as PBS-KD and PBS-WT. b, Representative photo shown NS2-KD and NS2-WT mice at the 8th day after in vivo leukemia transplantation. c and d, Body weight (c) and survival rate (d) of indicated mice. Each group contained 8-10 mice. Mice were monitored for a period of 15 days. e, Bioluminescent imaging was performed to examine distribution of GFP-positive NS2 cells in indicated mice. f, Percentage of GFP-positive NS2 cells in PBCs of indicated mice was detected by flow cytometry. Shown were representative images from at least three independent experiments. g, Shown were representative images of spleen from indicated mice by HE staining