Fig. 6
From: Novel lncRNA-IUR suppresses Bcr-Abl-induced tumorigenesis through regulation of STAT5-CD71 pathway
LncRNA-IUR-5 regulates STAT5-mediated CD71 expression. a, Transcriptome RNA sequencing analysis of lncRNA-IUR-5 knockdown K562 cell lines and control cells. Arrows indicated three isoforms of CD71. b, Western blotting analysis of CD71 levels in lncRNA-IUR-5 knockdown or overexpressing K562 cell lines. c-e, Western blotting analysis of CD71 levels in K562 cells upon imatinib treatment (c), STAT5-IN-1 (50 μM, 6 h) treatment (d), or knocking down STAT5 using sh-STAT5 (e). f, Western blotting and RT-PCR were performed to examine the protein and mRNA levels as indicated. g, Western blotting analysis of purified binding proteins with lncRNA-IUR-5, which were pulled down from K562 cell lines stably expressing S1, IUR-5 or IUR-5-S1. h, The interaction between lncRNA-IUR-5 and STAT5 was verified through RIP. lncRNA-BGL3 and GAPDH served as negative controls (n = 3; means ± SEM; *p < 0.05). i and j, Quantitative real-time PCR was performed to examine lncRNA-IUR expression in K562 cells upon STAT5-IN-1 (50 μM, 6 h) treatment (i) or knocking down STAT5 using sh-STAT5 (j) (n = 3; means ± SEM; **p < 0.01; *p < 0.05)