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Fig. 2 | Molecular Cancer

Fig. 2

From: m6A-induced lncRNA RP11 triggers the dissemination of colorectal cancer cells via upregulation of Zeb1

Fig. 2

RP11 triggers the dissemination of CRC cells both in vitro and in vivo. a CRC cells were transfected with the vector control or pcDNA/RP11 for 48 h, and proliferation was measured with a CCK-8 kit. b The wound healing of HCT-15 RP11 stable overexpression and control cells was recorded (left) and quantitatively analysed (right). c The in vitro invasion of HCT-15 RP11 stable overexpression and control cells was recorded (left) and quantitatively analysed (right). d The expression of EMT-related markers of HCT-15 or HCT-8 RP11 stable overexpression and control cells was verified by western blot analysis. e After transfection with si-NC or si-RP11 for 48 h, the expression of EMT markers in SW620 cells was verified by western blot analysis. f Tumour growth curves of HCT-15 RP11 stable overexpression and control cells in xenograft models at the indicated time intervals. g Weights of tumours derived from HCT-15 RP11 stable overexpression or control cells in xenograft models at the end of the experiment. h IHC analysis of Ki67-, vimentin- or fibronectin-stained paraffin-embedded sections obtained from xenografts. (I).HCT-15 RP11 stable overexpression and control cells were injected into nude mice via the tail vein. Representative images and H&E staining of metastatic liver tumours are shown. j The number of metastatic sites of tumours derived from HCT-15 RP11 stable overexpression or control cells was quantitatively analysed. Data are presented as the mean ± SD from three independent experiments. Bar = 200 μm. *p < 0.05, **p < 0.01 compared with control

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