Fig. 4

Downregulation of Siah1 and Fbxo45 mediates the RP11-induced upregulation of Zeb1. a & b The mRNA expression levels of 7 reported target proteins related to Zeb1 stability in HCT-15 a or HCT-8 (b) RP11 stable overexpression and control cells were determined by qRT-PCR. c The protein expression of Siah1 and Fbxo45 in HCT-15 or HCT-8 RP11 stable overexpression and control cells was determined by western blot analysis. d IHC analysis of Siah1- or Fbxo45-stained paraffin-embedded sections obtained from HCT-15 RP11 stable overexpression and control xenografts. e HCT-15 cells were transfected with vector control, pcDNA/RP11, pcDNA/Siah1, or Fbxo45 alone or together for 48 h, protein expression was verified by western blot analysis. f RIP-PCR was performed to analyse the relative enrichment of RP11 by use of an antibody against Siah1 or Fbxo45 in HCT-15 cells. Data are presented as the means ± SD from three independent experiments. Bar = 200 μm. **p < 0.01 compared with control