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Fig. 5 | Molecular Cancer

Fig. 5

From: Long non-coding RNA GBCDRlnc1 induces chemoresistance of gallbladder cancer cells by activating autophagy

Fig. 5

GBCDRlnc1 promotes autophagy of gallbladder cancer cells in vitro. a The protein levels of LC3 and p62 in Dox-resistant gallbladder cancer cells and their parental cells under different transfection were determined by western blot assay. b The protein levels of LC3 in Dox-resistant gallbladder cancer cells under different transfection with CQ (10 μM) were determined by western blot assay. c The protein levels of LC3 in the parental gallbladder cancer cells under different transfection with 3-MA (10 mM) were determined by western blot assay. d Autophagy was evaluated in Dox-resistant gallbladder cancer cells under different transfection using TEM. e Dox-resistant gallbladder cancer cells stably expressing stubRFP-sensGFP-LC3 under different transfection were observed by the fluorescence microscope. f The parental gallbladder cancer cells stably expressing stubRFP-sensGFP-LC3 under different transfection were observed by the fluorescence microscope

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Molecular Cancer

ISSN: 1476-4598