Fig. 7
Knockdown of PGK1 suppresses autophagy-associated chemoresistance of gallbladder cancer cells in vitro. a The protein levels of PGK1 in Dox-resistant gallbladder cancer cells and their parental cells were determined by western blot assay. b The sensitivities of NOZ/Dox cells under different transfection with Dox were determined by CCK-8 assay. c The protein levels of LC3 and p62 in Dox-resistant gallbladder cancer cells under different transfection were determined by western blot assay. d The protein levels of LC3 in NOZ/Dox cells under different transfection with CQ (10 μM) were determined by western blot assay. e Dox-resistant gallbladder cancer cells stably expressing stubRFP-sensGFP-LC3 under different transfection were observed by the fluorescence microscope. f The sensitivities of NOZ/Dox cells under different transfection with Dox were determined by CCK-8 assay. The protein levels of LC3 and p62 in Dox-resistant gallbladder cancer cells under different transfection were determined by western blot assay. g The protein levels of ATG3, ATG5, ATG7, ATG12 and Beclin1 in Dox-resistant gallbladder cancer cells under different transfection were determined by western blot assay. The mean ± SD of triplicate experiments were plotted, ***P < 0.001