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Fig. 3 | Molecular Cancer

Fig. 3

From: Function and clinical relevance of RHAMM isoforms in pancreatic tumor progression

Fig. 3

RHAMMB is upregulated in human pancreatic ductal adenocarcinoma (PDAC) and correlates with poor survival. a RHAMMA and RHAMMB expression values from TCGA PDAC dataset. RHAMMA: uc003lzf or NM_012484. RHAMMB: uc003lzg or NM_012485. cancer (ca): n = 124; control (ctrl): n = 4. Bars and error bars represent means and standard errors. b-d Kaplan-Meier survival analysis of TCGA cohort with 93 PDAC cases. High and low represent the status of the RHAMM mRNA expression levels compared to average values. e Knockdown efficiency of two EGFR shRNAs. S7 and S9 reduced EGFR protein expression and p-Erk1/2 levels in N134 cells overexpressing RHAMMB by Western blot analysis. α-tubulin was used a loading control. f EGFR knockdown greatly inhibited the liver metastasis of N134 cells overexpressing RHAMMB. A total of 1 million N134_RHAMMB_shLacZ, N134_RHAMMB_shEGFR(S7), or N134_RHAMMB_shEGFR(S9) cells were injected into the tail vein of NSG mice (n = 5 for each group). At the indicated time points, the recipient mice were euthanized to survey for metastatic sites and incidence. The number of liver macrometastases was recorded. g Western blot analysis of p-Erk1/2 and total Erk from N134 overexpressing luciferase (control), and N134_EGFR*. h N134 cells overexpressing luciferase (Luc), N134_EGFR* (EGFR*), N134_RHAMMB (RHAMMB) were injected into the tail vein of NSG mice (n = 5, each group). Five weeks later (for Luc and EGFR* groups) or when mice were lethargic (for RHAMMB), mice were euthanized to survey for metastatic sites and incidence. *: p < 0.0001, One-way ANOVA and pairwise comparison with Tukey’s adjustment

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