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Fig. 1 | Molecular Cancer

Fig. 1

From: CAFs secreted exosomes promote metastasis and chemotherapy resistance by enhancing cell stemness and epithelial-mesenchymal transition in colorectal cancer

Fig. 1

CAFs-derived exosomes promote invasion, metastasis, and chemotherapy resistance in CRC. a Transmission electron micrograph of NFs-derived exosomes (NFs-exos) and CAFs-derived exosomes (CAFs-exos). White arrow head points at exosomes. b Exosomes markers CD63, CD81 and TSG101 proteins were detected by western blot assay in NFs-exos, CAFs-exos, and the corresponding supernatant of NFs-CM and CAFs-CM obtained through ultracentrifugation. c Internalization of exosomes by SW480 cells examined by laser scanning confocal microscope. d Effect of NFs-CM, CAFs-CM, CAFs-exos, and CAFs-exos depleted of exosomes on migration and invasion of SW480, SW620 and LOVO cells by Boyden chamber assay. e Effect of NFs-CM, CAFs-CM, CAFs-exos, and CAFs-CM depleted of exos on the formation of metastasis nodules in lung. f-h Effect of NFs-CM, CAFs-CM, CAFs-exos, CAFs-CM depleted of exosomes on abilities of cell survival f, colony formation g and apoptosis h of SW480, SW620 and LOVO cells by CCK-8, colony formation and flow cytometry assays. i Effect of NFs-exos and CAFs-exos on percentage of CD133+CD44+ in SW480, SW620 and LOVO cells by flow cytometry assay. j-l Effect of NFs-exos and CAFs-exos on the expressions of stemness markers CD133, CD44, OCT4 j, k and EMT markers (l in SW480, SW620 and LOVO cells by real-time PCR and western blot assays

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Molecular Cancer

ISSN: 1476-4598