Fig. 2

Direct transfer of CAFs secreted exosomal miR-92a-3p to CRC cells. a Hierarchical clustering analysis of differentially expressing miRNAs among NFs-exos, CAFs-exos, SW480^NFs-exos and SW480^CAFs-exos using microRNA microarray. b Relative expression of miR-92a-3p, miR-181d-5p, miR-221–3p, miR-125b-5p, miR-185-5p and miR-625-3p in NF-exos, CAFs-exos, SW480^NFs-exos and SW480^CAFs-exos cells by real-time PCR assay. U6 was used as internal control. c Relative expression of miR-92a-3p in normal colorectal cell NCM460, CRC cell line cells, matched CRC tissues and normal colorectal mucosa, CAFs and NFs, CAFs-exos and NFs-exos from a same CRC patient by real-time PCR analysis. U6 was used as internal control. d Relative expression of miR-92a-3p in Blank, SW480^CAFs-exos, SW480^NFs-exos, SW620^CAFs-exos, SW620^NFs-exos, LOVO^CAFs-exos and LOVO^NFs-exos cells by real-time PCR assay. U6 was used as internal control. e & f Relative expression of miR-92a-3p e and pre-miR-92a-3p f in SW480^CAFs-exos, SW480^NFs-exos, SW620^CAFs-exos, SW620^NFs-exos, LOVO^CAFs-exos and LOVO^NFs-exos cells at indicated time by real-time PCR assay. U6 was used as internal control. g Relative expression of miR-92a-3p in SW480, SW620 and LOVO cells treated with antimiR-NC, antimiR-92a-3p, antimiR-92a-3p + NFs-exos, antimiR-92a-3p + CAFs-exos by real-time PCR analysis. U6 was used as internal control. h Transwell co-culture of SW480 cells with CAFs and CAFs/miR-92a-3p-FAM cells. Cultured SW480 cells were harvested and observed using laser scanning confocal microscope. i Incubation of SW480 cells with CAFs-exos and CAFs/miR-92a-3p-FAM secreted exosomes. Cells were observed using laser scanning confocal microscope