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Fig. 4 | Molecular Cancer

Fig. 4

From: CAFs secreted exosomes promote metastasis and chemotherapy resistance by enhancing cell stemness and epithelial-mesenchymal transition in colorectal cancer

Fig. 4

FBXW7 and MOAP1 attenuate miR-92a-3p-mediated promotion of aggressiveness and chemotherapy resistance of CRC. a Sequences of miR-92a-3p and the potential miR-92a-3p-binding sites at the 3’UTR of FBXW7 and MOAP1. Also shown are nucleotides mutated in FBXW7–3′-UTR mutant and MOAP1–3′-UTR mutant. Seed sequences are marked. b & c Effect of Blank, Mock and ectopic miR-92a-3p expression on the luciferase activity of FBXW7 3’UTR wild type b, FBXW7 3’UTR mutation c, MOAP1 3’UTR wild type b, and MOAP1 3’UTR mutation c in HEK293A, SW480, SW620 and LOVO cells by dual-luciferase reported assay. d&e Expression of FBXW7 and MOAP1 in SW480, SW620 and LOVO cells transfected with Mock, miR-92a-3p, miR-92a-3p/FBXW7, and miR-92a-3p/MOAP1 by real-time PCR d and western blot e assays. GAPDH was used as internal control. f Expression of FBXW7 and MOAP1 in SW480, SW620 and LOVO cells treated with NFs-exos, CAFs-exos, CAFs-exos/FBXW7, CAFs-exos/MOAP1 by western blot. GAPDH was used as internal control. g&h Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7 treatment on migration g and invasion h of SW480, SW620 and LOVO cells by Boyden chamber. i Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7, and miR-92a-3p/MOAP1 treatment on colony formation ability of SW480, SW620 and LOVO cells by plate colony formation assay. j & k Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7 j, miR-92a-3p/MOAP1 k treatment on survival of SW480, SW620 and LOVO cells by CCK-8 assay. l Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7 treatment on sphere formation of SW480, SW620 and LOVO cells by spheres formation assay. m Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7 transfection on the expression of CD133, CD44, OCT4, CyclinD1, C-myc, E-cad and N-cad expression in SW480, SW620 and LOVO cells by real-time PCR. GAPDH was used as internal control. n Effect of Mock, miR-92a-3p, miR-92a-3p/FBXW7 treatment on the expression of FBXW7, CD133, CD44, OCT4, E-cad and N-cad expression in SW480, SW620 and LOVO cells by western blot. GAPDH was used as internal control. o Effect of Mock, miR-92a-3p, miR-92a-3p/MOAP1 transfection on the expression of MOAP1, BAX and cleaved caspase3 in SW480, SW620 and LOVO cells by western blot. GAPDH was used as internal control

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