Fig. 7

The expression of miR-92a-3p correlates negatively with FBXW7 and MOAP1 and is associated with metastasis in serum exosomes. a Real-time PCR analysis of miR-92a-3p, FBXW7 and MOAP1 in non-metastatic and metastatic fresh CRC tissues n = 20 for each group) (tumor ration to normal). The expression of miR-92a-3p was compared between CRC with metastasis and CRC without metastasis. U6 was used as internal control. b & c Real-time PCR analysis of FBXW7 b and MOAP1 c in non-metastatic and metastatic fresh CRC tissues (n = 20 for each group) (tumor ration to normal). GAPDH was used as internal control. (D&E) Pearson correlation analysis between miR-92a-3p and FBXW7 levels, miR-92a-3p and MOAP1 levels in 20 cases of non-metastatic CRC d, 20 cases of metastatic CRC e and corresponding normal colorectal mucosa. f Real-time PCR analysis of miR-92a-3p in serum-derived exosomes collected from 30 normal persons, 30 non-metastatic CRC patients and 30 metastatic CRC patients. U6 was used as internal control. g Real-time PCR analysis of miR-92a-3p in serum-derived exosomes collected from 18 cases of 5-FU/L-OHP sensitive CRC patients and 18 cases of 5-FU/L-OHP resistant CRC patients. U6 was used as internal control. h Briefly, Colorectal cancer (CRC) cells uptake cancer associated fibroblasts secreted exosomes, leading to an increase of miR-92a-3p and stemness, EMT, metastasis, and 5-FU/L-OHP resistance in CRC cells. Mechanically, miR-92a-3p promotes aggressiveness and chemotherapy resistance by directly binding to 3’UTR of FBXW7 and MOAP1 and suppressing their expressions in CRC cells. Re-expression of FBXW7 and MOAP1 attenuate the role of miR-92a-3p by inhibiting Wnt/β-catenin and mitochondrial apoptosis in CRC