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Fig. 3 | Molecular Cancer

Fig. 3

From: MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC

Fig. 3

MED12 knockout caused a multinucleation phenotype in NSCLC cells. a-b HE staining showed the multinucleation in MED12 WT, MED12 KO and MED12 reconstitution single clones of PC9 and SPC-A1 cells. Arrowheads indicated multinucleated cells. Scale bar: 100 μm. *P < 0.05. c Expression of active β-galactosidase detected by X-gal staining. β-gal positive cells represent senescent cells. Scale bar: 100 μm. d Representative photograph of multinucleated cells sorted from MED12 KO1 of PC9 cells. Scale bar: 100 μm. e Cell counting assay in WT, KO1 and multinucleated cells sorted from MED12 KO1 of PC9 cells. f The survival of MED12 WT and multinucleated cells sorted from MED12 KO1 of PC9 cells in RPMI-1640 and conditional reprogramming (CR) medium. Scale bar: 100 μm. g Schematic of xenograft experiment in vivo. h Representative photographs of xenograft tumors at the end of the experiment

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